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KMID : 1007520170260041013
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Food Science and Biotechnology
2017 Volume.26 No. 4 p.1013 ~ p.1018
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Effect of sodium selenite on the bacteria growth, selenium accumulation, and selenium biotransformation in Pediococcus acidilactici
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Kousha Masoud
Yeganeh Sakineh
Amirkolaie Abdolsamad Keramat
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Abstract
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In this study, the effect of low selenium concentrations on bacteria growth, selenium bioaccumulation, and selenium speciation in Pediococcus acidilactici was investigated. Six different sodium selenite (Na2SeO3) solutions with concentrations of 0, 0.5, 1, 2, 3, and 4 mg/L were added in MRS broth for 24 h. Then, the obtained bacterial pellets were weighed. The contents of total selenium and selenium species in the bacterial pellets were measured via optimized enzymatic hydrolysis and HPLCICP-MS. The maximum dried P. acidilactici biomass of 1.44 g/L was achieved by utilizing 1 mg/L Na2SeO3. By increasing sodium selenite concentrations, total selenium contents were significantly increased from 0.14 to 1.45 mg/ g dry weight (p＜0.05). The findings indicated that selenium was favorably incorporated into the bacteria protein fraction and mainly formed selenocysteine. Therefore, selenium-enriched lactic acid bacterium P. acidilactici can deliver a less-toxic, more bioavailable selenium source for human and animal nutrition.
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KEYWORD
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Selenium (Se), Sodium selenite (Na2SeO3), Lactic acid bacteria (LAB), Pediococcus acidilactici, Enrichment
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